synthesis of human placental cdna and demonstration of the expression of m-csf in that tissue

macrophage colony stimulating factor (m-csf) has previously been shown to affect the differentiation of cells of the mono-nuclear phagocytic line. more recent studies indicate that m-csf may have a role in pregnancy. in the present study, the expression of m-csf in the human placenta was demonstrated. placental mrna was isolated and used as template for synthesis of complementary dna (cdna). the presence of m-csf related sequences in the cdna was shown by pcr and rt-pcr reactions in which m-csf specific primers were used. in addition, it was shown that a 2.4 kb cdna after electrophoresis and transfer to a nylon filter, hybridized with a digoxygenin labeled m-csf specific probe.